The analysis of expression of p16 protein in group of 53 patients treated for sinonasal inverted papilloma.

INTRODUCTION: Sinonasal inverted papilloma constitute relevant therapeutic problem due to destructive character of growth, tendency to recur and the possibility of malignant transformation. Therefore, many attempts to identify risk factors for inverted papilloma occurrence have been undertaken, as well as research to find markers that would allow for the earlier detection of tumors and the application of adequate therapy. A widely known risk factor of inverted papilloma is HPV infection. One of the markers of HPV infection and the ongoing effect of this change (although arousing some controversy) is the expression of the p16 protein. OBJECTIVE: The aim of the study was to analyze the correlation between the expression of p16 as a surrogate of HPV infection in analyzed histopathological material and epidemiological variables, recurrences or malignant transformation. METHODS: The retrospective study includes a group of 53 patients (18 women and 35 men) undergoing treatment for sinonasal inverted papilloma in the period of 2002-2012. The intensity of the p16 protein in histopathological material was scored as: 0 - no expression, 1 - diffuse expression (borderline) and 2 - positive expression; or 0 - no expression/diffuse expression (borderline); 1 - positive expression. The Ethics Committee agreement was obtained (1089/12; 245/13). RESULTS AND CONCLUSION: There was no statistically significant relationship between the expression of p16 and the age of patients, cigarette smoking, tumor location, tumor staging according to the Krouse and Cannady classification, the presence of dysplasia or the occurrence of relapse.

The rapid change in sinonasal inverted papilloma incidence - the epidemiological analysis.

In recent years, the incidence of sinonasal inverted papilloma (IP) in a stable population has increased significantly. The aim of the study was to analyze the occurrence of IP between the years 2002-2012 in relation to epidemiological factors in an unalterable area. Moreover, we wanted to start a discussion about the difficulties in determining the inci¬dence of benign lesions that are not included in registers. This retrospective study included a group of 69 patients who were treated in the Department of Otolaryngology, ENT Oncology, Poznan University of Medical Sciences. In 2006, we noticed a sudden change in referrals. We compared two groups of patients who were referred during two differ¬ent time periods: 11 patients (2002-2006) and 58 patients (2007-2012). Despite a marked difference in the number of patients, there were no differences between the groups in tumor location (p>0.05), stage (p=0.16), duration of symp¬toms (p=0.39), place of residence, and smoking (p=0.41). The patient`s age was the only variable that differed significantly between the groups (48.45 vs. 56.93 years; p=0.043569, respectively).

Clinical outcomes of treatment of sinonasal inverted papillomas (IPs) depending on the surgical technique and learning curve.

INTRODUCTION: Sinonasal inverted papillomas (IPs) are a major challenge for ENT specialists. In the last decades, the number of endoscopic procedures has increased, while the percentage of external or double approach procedures has decreased. The aim of this study was to evaluate long-term IP treatment outcomes according to the applied surgical method. MATERIAL AND METHODS: the retrospective study included 69 patients (28 women and 41 men) treated during 2002-2012 in the Department of Otolaryngology and Laryngological Oncology of Poznan University of Medical Sciences. Of these patients, 47 were operated endoscopically, 16 received a double procedure and six received an external procedure. RESULTS: The recurrence rate in patients treated endoscopically was 32%, while patients treated by the external/double approach showed 64% (p = 0.03306). The recurrence rate after endoscopic surgery was 75% during 2002-2006, but was 28% during 2007-2012. There was no correlation between recurrence rate and age (p = 0.087686), gender (p = 0.42810), type of symptoms (p = 0.20955), or Krouse staging (p = 0.23658). A significant impact on relapses was the position to Ohngren's plane (p = 0.004768) and tumor location in the anterior ethmoid (p = 0.00416). DISCUSSION: Endoscopic procedures are an effective method of IP treatment, however reasonable indications and surgeon experience should be taken into consideration.